Quantitation of Embelin from Plant Embelia Drupacea (dennst) M. R & S. M. Almeida by Development and Validation of H.p.t.l.c. Method

Fruits of Embelia drupacea known locally as Ambat are traditionally employed as laxative, antiseptic and antihelmintic and could be use as substitute for Ayurvedic drug ‘Vidanga’ Embelia ribes Burm F. ‘Vidanga’ contains as many as 35 bioactive compounds of which Embelin is most important one. Quntitation of embelin from fruits of E. drupacea also belonging to family Myrsinaceae by development and validation of H.P.T.L.C method has been demonstrated in this communication. For estimation of Embelin from E. drupacea fruits a selective, precise and accurate High Performance Thin Layer Chromatography (H.P.T.L.C) method has been developed. The method developed involved TLC aluminium plate (20×10 with thickness 250 μm) precoated with Silica gel 60 F254 as stationary phase with solvent system Chloroform: Ethyl acetate: Formic acid (5:4:1 v/v/v). Densitometric analysis was carried out in the absorbance mode at 348 nm using Camag TLC Scanner – IV. The developed HPTLC method showed good regression (r = 0.99795 ± 0.0020) and the recovery of Embelin was in the range of 97% 113%. The limit of detection and limit of quantitation were found to be 0.69 ng / spot and 2.11 ng / spot respectively. The method was validated for precision, recovery, limit of detection, limit of quantitation and linearity. H.P.T.L.C. quantitation was done at multilevel (for all concentrations). The developed method was found to be simple, precise and accurate and could be used for the quality control of the raw material as well as formulations.